Prep HPLC Column, UDA (wcx), 4um, 100A. 30mm ID x 250mm Length, Preparative. Cogent TYPE-C Brand. 1 EA.
Additional Info:
40031-P30-250 Cogent UDA™ (weak cation exchange, WCX) Preparative HPLC Column is built on Cogent TYPE‑C™ silica (silica hydride platform), delivering one of the most versatile and powerful separation mechanisms available for preparative chromatography.
The UDA™ ligand (Undecanoic Acid) consists of an 11‑carbon hydrophobic chain with a terminal carboxylic acid group, providing true multi‑mode chromatographic functionality:
Reversed phase (hydrophobic retention)
Weak cation exchange (active at pH ≥ 6)
HILIC (polar interaction capability)
This unique mixed‑mode behavior enables superior selectivity for polar, ionizable, and basic compounds, making it especially effective for large‑scale purification of complex matrices where traditional C18 or ion exchange columns cannot resolve co‑eluting components.
The TYPE‑C silica hydride base provides key performance advantages:
Faster equilibration between runs
Reduced sensitivity to moisture in mobile phases
Exceptional chemical stability (including low pH <2 operation)
Extremely robust column lifetimes under heavy preparative use
Minimal salt requirements in HILIC mode (cleaner fractions and improved downstream processing)
250 mm Length – Maximum Resolution for Large‑Scale Purification
The 250 mm length delivers the highest resolving power in the UDA™ preparative column family.
Maximum resolution compared to 50 mm, 100 mm, and 150 mm columns
Superior separation of closely related or co‑eluting compounds
Highest peak capacity for very complex purification challenges
Extended retention for improved isolation purity
✅ Recommended for final purification, impurity isolation, and high‑purity production workflows
Flow Rate & System Requirements (30 mm ID, 4 µm)
⚠️ Requires preparative or process‑scale HPLC system capability
Typical flow rate range: ~25 – 60 mL/min
Increased backpressure due to longer column length, but manageable
Designed for very high‑flow, large‑volume purification
Compatible with industrial fraction collection systems
✅ Important: A specialized preparative or process‑scale HPLC system is required for columns of this size
✅ Best Practice: Use proper sample preparation and a matched guard column to protect the column and ensure consistent long‑term performance
Unique Selectivity – Why Choose UDA™ (WCX)
True Mixed‑Mode Retention
Hydrophobic (RP)
Ionic (weak cation exchange)
Polar (HILIC)
Powerful for Complex Purifications
Resolves co‑eluting and structurally similar compounds
Highly effective for basic and positively charged analytes
pH‑Tunable Selectivity
WCX mechanism active at pH ≥ 6
Retention optimized via pH and ionic strength adjustments
✅ Ideal when traditional preparative C18 or ion exchange columns fail to achieve required resolution or purity
TYPE‑C Silica Advantages
Faster Equilibration
Maximizes throughput in production environments
No Strict Moisture Control (HILIC)
Simplifies method setup and solvent handling
Robust Column Lifetimes
Engineered for repeated, large‑scale use
Low Salt Elution (HILIC Mode)
Cleaner fractions and improved product recovery
Cogent UDA™ Column Advantages
Multi‑Mode Flexibility
RP, HILIC, and WCX in one column
Maximum Resolution Capability
Ideal for the most challenging purification tasks
Ultra High‑Capacity Prep Scale (30 mm ID)
Supports large‑volume injections and production workflows
Optimized for Complex Separations
Exceptional performance in difficult matrices
Typical Applications
Large‑scale preparative HPLC purification
Process‑scale production and isolation
Impurity isolation and final product purification
Complex matrix separations
Basic, polar, and ionizable compound purification
Important Usage Notes
Packed and shipped in reversed‑phase solvents
Requires preparative or process‑scale HPLC system capability
Designed for very high‑flow operation (30 mm ID columns)
Increased pressure due to extended column length
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable